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KMID : 1101320120440020081
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Korean Journal of Clinical Laboratory Science
2012 Volume.44 No. 2 p.81 ~ p.85
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Phenotypic and Genotypic Detection of Metallo-ß-Lactamase Producing Pseudomonas aeruginosa
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Yang Byoung-Seon
Hong Keun-Seok
Jung Seung-Bong
Kwon Young-Hoon
Jeong Jong-Yoon
Lee Min-Joo
Lee Hye-In
Park Mi-Seon
Choi Seung-Gu
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Abstract
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This study was undertaken to evaluate phenotypic and genotypic methods for detection of Metallo- Beta-Lactamases (MBLs) among nosocomial Pseudomonas aeruginosa. Of the 50 P. aeruginosa isolates from clinical specimens, 20 were evaluated for carbapenem resistance and screened for MBL by doubledisk synergy test and combined-disk test. Nineteen strains (95%) were found to be MBL producers among the 20 P. aeruginosa. MBL positives were further confirmed by Polymerase Chain Reaction (PCR). For the IMP and VIM types of MBLs, PCR analysis was performed on 19 of the 20, and 10 were positive for VIM MBL type. This study reports the validation of a simple and accurate MBL detection method that can be easily incorporated into the daily routine of a clinical laboratory. Early detection of MBL-carrying organisms, including those with susceptibility to carbapenems, is of paramount clinical importance, as it allows rapid initiation of strict infection control practices as well as therapeutic guidance for confirmed infection.Key Words : Hepatitis A virus (HAV), Anti-HAV, Hospital workers, Prevalence, Vaccination.
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KEYWORD
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Metallo-ß-Lactamases, Double-disk synergy test, Combined-disk test
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